Table 1 Plasmids and strains used in this study

TOP10

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(Str^R) endA1 λ^-

Invitrogen

DB3.1

F- gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(r_B^-, m_B^-) ara14 galK2 lacY1 proA2 rpsL20(Sm^r) xyl5 Δleu mtl1

Invitrogen

DH5alpha

F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ^-

Invitrogen

MG1655

F- λ- ilvG- rfb-50 rph-1

CGSC

Plasmids

Name

BioBrick™ code

Description

pLC-ccdB

pSB4C5(BBa_I52002)^a

pUC19-derived pMB1 replication origin and ccdB toxin constitutive expression cassette in low copy vector

pHC-T4Lys^-

pSB1A2(BBa_K112808)^a

promoterless lysis device in high copy vector

pHC-HSL

pSB1A2(BBa_F2620)^b

HSL-inducible promoter in high copy vector

pHC-RFP

BBa_J61002(BBa_J23118)^a

RFP constitutive expression cassette

pHC-Heat

pSB1A2(BBa_ K098995)^a

heat-inducible promoter in high copy vector

pHC-T4LysHSL

pSB1A2(BBa_K173015)^c

HSL-inducible lysis device in high copy vector

pLC-HSL

pSB4C5(BBa_F2620)^c

HSL-inducible promoter in low copy vector

pLC-T4Lys^-

pSB4C5(BBa_K112808)^c

promoterless lysis device in low copy vector

pLC-T4LysHSL

pSB4C5(BBa_K173015)^c

HSL-inducible lysis device in low copy vector

pLC-T4LysHeat

pSB4C5(BBa_J107014)^c

heat-inducible lysis device in low copy vector