Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

Table 2 Quantitative characterization of TOP10 bearing the HSL-inducible lysis device in low copy plasmid grown at 37°C in microplate

	TOP10 with pLC-T4LysHSL
	Exponential phase	Early stationary phase	Late stationary phase
Lysis entity [%]	76.28 ± 0.3	75.43 ± 1.1	50.1 ± 2.5
Lysis delay after induction [min]	15	15	15
Doubling time [min]	49.8 ± 1.1
Doubling time of negative control [min]	43 ± 1.3

Induction was carried out with HSL 100 nM. Mean lysis entity and lysis delay after the induction are reported for the different growth phases together with their standard error, measured on 3 independent experiments. The doubling time of the uninduced lysis device and its negative control are reported too.

ISSN: 1754-1611