Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

Table 3 Quantitative characterization of TOP10 bearing the thermoinducible lysis device in low copy plasmid grown at 30°C in microplate

TOP10 with pLC-T4LysHeat
Lysis entity in exponential phase [%]	73 ± 0.3
Lysis delay after induction [min]	55
Doubling time [min]	66.1 ± 1.7
Doubling time of negative control [min]	65.9 ± 0.6

Induction was carried out by shifting the temperature from 30°C to 42°C. Mean values estimated on three wells in the same experiment are reported with standard errors.

ISSN: 1754-1611