Figure 5
Hypothetical combinatorial DeviceEditor designs. (A) Metabolic pathway library: 29 bins and 19 unique part icons (several repeated through the design). Clicking on a repeated part icon, here "RBS_pET29_5'" (top left), highlights all replicates with thick blue outlines. (B) Gene over-expression library (rotated 90° counter-clockwise): 8 bins and 156 part icons (none repeated). Note that there are only 38 barcodes (sixth bin) that correspond one-to-one with the 38 vector backbones (first bin). This design allows for a single short sequencing read spanning the end of gene 2 (fourth bin) through the barcode and the beginning of gene 3 (eighth bin) to uniquely identify a plasmid combination. As a result, there is not a unique barcode required for each of the 383 = 54,872 possible plasmid combinations. Zoom in with PDF display software to improve legibility.