Figure 1

Degradation of ssrA-tagged and untagged LacI at 25°C and 37°C. This figure shows representative western blots and quantification of the levels of LacI at 0 minutes (prior to the addition of chloramphenicol) and 10-60 minutes after addition of chloramphenicol in E. coli M182 grown at 25°C (squares) and 37°C (diamonds). Sample loading was normalised by culture OD. LacI levels are shown relative to the amount present at t=0, are the mean of three independent experiments and are shown with standard error.