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Figure 3 | Journal of Biological Engineering

Figure 3

From: Development of a modified Respiration Activity Monitoring System for accurate and highly resolved measurement of respiration activity in shake flask fermentations

Figure 3

Oxygen transfer rates (OTR) and calibration factors (K) of cultures of E. coli BL21 pRSET eYFP-IL6 in mineral medium measured with the RAMOS device by applying both the method of Anderlei et al.[23, 24]and the newly developed evaluation method. A: OTR calculated by applying the evaluation method of Anderlei et al.[23, 24] using equation A.2 (black triangle) and the corresponding calibration factor K calculated with equation A.3 (red triangle); B: OTR calculated by applying the new evaluation method using equation (B.1) in the rinsing phase (open square), equation (B.4) in the stop phase (black square) and K, calculated as illustrated in Figure2 and equation (B.7) (red square) with its smoothing spline fit (red line); experimental conditions: Wilms-MOPS medium with 20 g/L glucose; temperature T = 30°C; filling volume VL = 10 mL; shaking diameter d0 = 50 mm; shaking frequency n = 350 rpm; initial optical density OD600 = 0.2; initial pH = 7.5; rinsing phase trinse = 25 min; stop phase tstop = 5 min; sensor lag time τ = 0.013 h.

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