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Figure 5 | Journal of Biological Engineering

Figure 5

From: Development of a modified Respiration Activity Monitoring System for accurate and highly resolved measurement of respiration activity in shake flask fermentations

Figure 5

Oxygen transfer rates (OTR) of E. coli BL21 pRSET eYFP-IL6 in mineral medium with different initial sorbitol concentrations. A: 0.5 g/L sorbitol; B: 1 g/L sorbitol; C: 1,5 g/L sorbitol; D: 2 g/L sorbitol; RAMOS data from the rinsing phase calculated with equation (B.1) (open square) and from the stop phase calculated with equation (B.4) (black square); glucose concentration (blue triangle); sorbitol concentration (orange circle); acetate concentration (green diamond); optical density (OD600) at 600 nm (red open circle); experimental conditions: Wilms-MOPS medium with 20 g/L glucose and different sorbitol concentrations; temperature T = 30°C; filling volume VL = 10 mL; shaking diameter d0 = 50 mm; shaking frequency n = 350 rpm; initial optical density OD600 = 0.2; initial pH = 7.5; rinsing phase trinse = 25 min; stop phase tstop = 5 min; sensor lag time τ = 0.013 h.

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