Figure 6

Comparison of the method of Anderlei et al.[23, 24]using equation (A.2) ( blue triangle ) and the new evaluation method using equation (B.1) in the rinsing phase ( open square ) and equation (B.4) in the stop phase ( black square ). A: Enlarged section of the OTR curve of Figure5A; B: E. coli BL21 pRSET eYFP-IL6 in TB medium; temperature T = 30°C; filling volume V_L = 10 mL; shaking diameter d₀ = 50 mm; shaking frequency n = 300 rpm; inoculated with 0.2 ml cryo culture; rinsing phase t_rinse = 55 min; stop phase t_stop = 5 min; high flow phase t_high = 0.9 min; C: Gluconobacter oxydans 621 H wildtype in complex medium with 40 g/L mannitol and 0.5 g/L glycerol; temperature T = 30°C; filling volume V_L = 10 mL; shaking diameter d₀ = 50 mm; shaking frequency n = 350 rpm; initial optical density OD₆₀₀ = 0.1; rinsing phase t_rinse = 25 min; stop phase t_stop = 5 min; a sensor lag time of τ = 0.013 h is used for all experiments.