Figure 3
From: Portable bacterial identification system based on elastic light scatter patterns
(a) Spatial filtering and colony counting. Raw plate image captured by the imaging camera. (b) Separation of colonies by subtracting the pre-saved background image and applying threshold. (c) Identification of colonies to be scanned by marking with blue color. (i) Colonies with too small or large diameters; (ii) colonies with low roundness values (indicating two or more intersecting colonies); (iii) colonies too close to the black hole in the raw plate image.