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Table 1 Validation of the Constructor by comparing five 2011 iGEM team cloning approaches to the recommendation by the Constructor

From: The Constructor: a web application optimizing cloning strategies based on modules from the registry of standard biological parts

iGEM 2011 team

iGEM project title

# of TUs in the designed circuit

# of used Biobrick Parts

Processing time (min)

   

Used by team

Recommended by the Constructor

 

KAIST-Korea1

E. casso

2

11

7

< 1 min

UANL_Mty-Mexico2

S.C.I.E.N.C.E. : Simple Code Interpretation Enabling Circuit in E. coli

7

26

25

674 min

XMU-China3

i-ccdB: intelligent Control of Cell Density in Bacteria

3

6

6

< 1 min

NTNU-Trondheim4

Red Fluorescent Stress Sensor

2

5

4

< 1 min

Wageningen UR5

The Synchroscillator: a Synchronized Oscillatory System

4

7

6

< 2 min

  1. 1http://2011.igem.org/Team:KAIST-Korea.
  2. 2http://2011.igem.org/Team:UANL_Mty-Mexico.
  3. 3http://2011.igem.org/Team:XMU-China.
  4. 4http://2011.igem.org/Team:NTNU_Trondheim.
  5. 5http://2011.igem.org/Team:Wageningen_UR.
  6. In these examples, the gene circuits contained between 2 and 7 Transcription Units (TUs).
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Journal of Biological Engineering

ISSN: 1754-1611