Figure 1

Normalized transfection profile for cells plated on surfaces with defined surface chemistries transfected with (a) Lipofectamine 2000 and (b) PEI. NIH/3T3 cells were seeded on SAM modified gold and tissue culture polystyrene control surfaces, nonviral DNA complexes with DNA encoding for EGFP and LUC were delivered 18 h later, and transfection profiles were acquired 48 h following complex delivery by quantifying the luciferase expression and normalizing these values per total protein amount present on the evaluated surfaces. Data is reported as mean +/− standard error of the mean of transfection profile values reported in relative light units (RLU) per mg protein. (*p < 0.05, **p < 0.01).