Figure 4

Confocal microscopy images of NIH/3T3 fibroblasts stained with TRITC phalloidin for actin filaments (red) and nuclei counterstained with DAPI (blue) on (a) -COO^-terminated, (b) -CH ₃ terminated, and (c) PS surfaces. Scale bar = 50 μm. Quantitative image analysis of (d) cytoskeletal stress bundles and (e) focal adhesions of NIH/3T3 fibroblasts on different biomaterial surfaces. Actin stress bundles were quantified by counting bundled actin fibers identified as bright regions of the TRITC stain, while focal adhesions were quantified by counting the amount of lamellipodial protrusions, which have previously been shown to directly correlate to focal adhesion abundance. Data is reported as mean +/− standard error of the mean (*p < 0.05, ***p < 0.001).