Table 1 Plasmids used in this study
Plasmids | Relevant characteristic(s) | Source / Reference |
|---|---|---|
pGEM-T easy | Cloning vector, Apr lacZ′, mcs | Promega |
pRL271 | Cloning vector carrying sacB, Em and Cm | GenBank accession #L05081 |
pUC4K | Source of Nm cassette | Amersham |
pRL632 | Helper plasmid carrying metylates AvaI, AvaII and AvaIII sites | [27] |
pRL443 | Conjugative plasmid, Km spontaneous mutant of RK2 | [27] |
pGhupS | pGem-T easy vector contained hupS | This study |
pGhupSNm | Nm cassette inserted into Eco RV site within hupS of pGhupS | This study |
pRLhupSNm | Cloning vector, Apr lacZ′, mcs | This study |