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Table 1 Plasmids used in this study

From: Inactivation of uptake hydrogenase leads to enhanced and sustained hydrogen production with high nitrogenase activity under high light exposure in the cyanobacterium Anabaena siamensis TISTR 8012

Plasmids Relevant characteristic(s) Source / Reference
pGEM-T easy Cloning vector, Apr lacZ, mcs Promega
pRL271 Cloning vector carrying sacB, Em and Cm GenBank accession #L05081
pUC4K Source of Nm cassette Amersham
pRL632 Helper plasmid carrying metylates AvaI, AvaII and AvaIII sites [27]
pRL443 Conjugative plasmid, Km spontaneous mutant of RK2 [27]
pGhupS pGem-T easy vector contained hupS This study
pGhupSNm Nm cassette inserted into Eco RV site within hupS of pGhupS This study
pRLhupSNm Cloning vector, Apr lacZ, mcs This study