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Figure 2 | Journal of Biological Engineering

Figure 2

From: A synthetic biology approach to self-regulatory recombinant protein production in Escherichia coli

Figure 2

Integration and testing of the circuit components. (A) Integration of a TetO site downstream of the T7 promoter on pET-(23). Promoter highlighted in green and TetO site in red. The diagram depicts the expression of GFP from the pET-(23) vector (grey bars) and the TetO-site containing pET23b_TetO plasmid (black bars). (B) Effect of different arabinose concentrations on GFP expression in cells containing a plasmid encoding TetR under the control of the arabinose operon promoter and either the pET-(23) plasmid (grey bars) or pET-23_TetO plasmid (black bars) for GFP expression. %ara - arabinose concentration (% w/v). (C) Increased cellular stress in GFP expressing cells. Expression of GFP from pET23b_TetO and RFP expression using the PIbpAB on a 2nd accessory vector. RFP expression in cultures induced for GFP expression (grey bars) and non-induced cultures (black bars) is shown. All data represent averages of 4 biological replicates +/- standard error of the mean.

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