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Figure 6 | Journal of Biological Engineering

Figure 6

From: Directed evolution of bright mutants of an oxygen-independent flavin-binding fluorescent protein from Pseudomonas putida

Figure 6

SDS PAGE analysis for wild type and mutant FbFPs. Lane 1: F37W, Lane 2: F37E, Lane 3: F37Y, Lane 4: F37T, Lane 5: F37S, Lane 6: Wild type FbFP. Selected mutants (F37W, F37E, F37Y, F37S, and F37T) of the FbFP F37X saturation mutagenesis library were purified and run on a 10% denaturing polyacrylamide gel (160 V, 45 minutes). Fluorescence enhanced mutants FbFP F37S and F37T comigrate with the wild type band (WT) at an approximate molecular mass of 17 kDa. Additional mutants correspond to neutral (F37E, lane 2) or deleterious mutants (F37W, lane 1 and F37Y, lane 3).

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Journal of Biological Engineering

ISSN: 1754-1611