Figure 8From: Directed evolution of bright mutants of an oxygen-independent flavin-binding fluorescent protein from Pseudomonas putidaEmission spectra of purified F37Y and F37W mutant proteins. F37Y and F37W mutants show severely quenched fluorescence emission yields at 500 nm relative to the wild type protein. Samples were excited at 450 nm, and all values were normalized to protein concentrations as determined by the Bradford assay.Back to article page