Figure 4

Growth kinetics of plasmid-containing strains. Maximum specific growth rate (μMax) was measured in the presence and absence of thiamphenicol at a range of temperatures for 6 strains of C. thermocellum containing potential temperature-sensitive plasmids and 2 control strains (plasmid pMU102 and no-plasmid). A solid black line indicates μMax in the absence of thiamphenicol. A solid red line indicates μMax in the presence of thiamphenicol. The dashed black line represents μMax of the no-plasmid control in the absence of thiamphenicol. The dashed red line represents the μMax of the positive control plasmid pMU102 in the presence of thiamphenicol. Error bars represent a standard deviation, n = 4.