Figure 6From: Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophilesRate of plasmid curing. Colony forming units (CFUs) were measured with and without thiamphenicol both at the beginning and end of growth in the absence of selection at 48°C and 55°C. These values were used to calculate the plasmid stability ratio (described in the text). A plasmid stability ratio of 1 implies stable replication. A plasmid stability ratio of 0 implies no replication. Error bars represent one standard deviation, n = 3.Back to article page