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Figure 1 | Journal of Biological Engineering

Figure 1

From: A BioBrick compatible strategy for genetic modification of plants

Figure 1

A standardized, modular system for the production of genetically-modified plants. Genetic parts (such as those obtained from the BioBrick Registry) were assembled and inserted into modified vectors (Open, Expression, or Reporter) in E. coli. These parts may be assembled to build constructs to impact a wide variety of plant phenotypes. Once assembled, these vectors were transformed into Agrobacterium. Using the floral dip procedure, Agrobacterium infected Arabidopsis, thereby transferring the assembled construct. Once seeds were produced, they were plated on selective media to obtain transgenic plants carrying the assembled construct.

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