Figure 2

CI turning on P _RM . (a) A two plasmid system was used to test the ability of P _RM activation by CI. This circuit consists of an IPTG inducible plasmid that expresses CI and a reporter plasmid expressing GFP by activation of P _RM . Two different ssrA tags were added to the CI protein (ec-ssrA, mf-ssrA) to test the effect of ssrA tags on P _RM activation. (b) Fluorescence measurements were normalized to OD₆₀₀ to account for cell density changes. Strains were cultured in various concentration of IPTG to test for P _RM activation. Error bars denote the measurement range (highest and lowest value). All data points were taken with three biological replicates.