Table 1 List of Plasmids (accession numbers are listed in the supplemental section S.5)
From: A genetic bistable switch utilizing nonlinear protein degradation
Name | Description | Marker | Origin |
|---|---|---|---|
PRM-GFP | GFP reporter expressed from P RM | Amp | p15A |
PLtetO-1-GFP | GFP reporter expressed from P LtetO-1 | Amp | p15A |
PLlacO-1-CIwt | IPTG inducible CI-wt | Cm | ColE1 |
PLlacO-1-CILVA | IPTG inducible CI-ec- ssrA | Cm | ColE1 |
PLlacO-1-CImf | IPTG inducible CI-mf-ssrA | Cm | ColE1 |
RFP1 | P LtetO-1 mf-lon (trunc) mCherry with RBS1 | Amp | p15A |
RFP2 | P LtetO-1 mf-lon (trunc) mCherry with RBS2 | Amp | p15A |
RFP3 | P LtetO-1 mf-lon (trunc) mCherry with RBS3 | Amp | p15A |
RFP4 | P LtetO-1 mf-lon (trunc) mCherry with RBS4 | Amp | p15A |
RFP5 | P LtetO-1 mf-lon (trunc) mCherry with RBS5 | Amp | p15A |
RFP6 | P LtetO-1 mf-lon (trunc) mCherry with RBS6 | Amp | p15A |
RFP7 | P LtetO-1 mf-lon (trunc) mCherry with RBS7 | Amp | p15A |
SW1 | Full switch with 5-UTR1 | Kan | pSC101 |
SW2 | Full switch with 5-UTR2 | Kan | pSC101 |
SW3 | Full switch with 5-UTR3 | Kan | pSC101 |
SW4 | Full switch with 5-UTR4 | Kan | pSC101 |
SW5 | Full switch with 5-UTR5 | Kan | pSC101 |
SW6 | Full switch with 5-UTR6 | Kan | pSC101 |
SW7 | Full switch with 5-UTR7 | Kan | pSC101 |
PRM-CIwt-TetR | CI-wt and TetR expressed from P RM | Kan | pSC101 |
PRM-CILVA-TetR | CI-ec-ssrA and TetR expressed from P RM | Kan | pSC101 |
PRM-CImf-TetR | CI-mf-ssrA and TetR expressed from P RM | Kan | pSC101 |
PLlacO-1-mfLon | IPTG inducible mf-Lon | Cm | ColE1 |