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Table 1 Promoter comparisons in sequence, regulation, induction and thermal opening probability pattern

From: Wide-dynamic-range promoters engineered for cyanobacteria

Promoter

-10 element and its downstream 8 basesa

Regulationb

Inductionc

Patternd

  

Induced

Repressed

  

L15

TATAAT G GA CACT

20.1 ± 0.1

0.243 ± 0.003

79 ± 1

A 〈17.9 ± 0.2〉

L07

TATAAT G GT CACT

19.8 ± 0.1

0.289 ± 0.003

65 ± 1

 

L05

TATAAT G CT CACT

16.3 ± 0.1

0.198 ± 0.003

78 ± 1

 

L13

TATAAT G CA CACT

15.5 ± 0.1

0.219 ± 0.003

67 ± 1

 

L03

TATAAT G GC CACT

19.2 ± 0.1

0.220 ± 0.003

83 ± 1

B 〈16.3 ± 0.2〉

L11

TATAAT G GG CACT

19.1 ± 0.1

0.236 ± 0.003

77 ± 1

 

L09

TATAAT G CG CACT

15.6 ± 0.1

2.88 ± 0.01

5.15 ± 0.01

 

noLVA_L09e

TATAAT G CG CACT

11.53 ± 0.05

0.545 ± 0.004

20.1 ± 0.1

 

L01

TATAAT G CC CACT

11.30 ± 0.05

0.235 ± 0.003

46 ± 1

 

L02

TATAAT G TC CACT

17.7 ± 0.1

0.214 ± 0.003

79 ± 1

C 〈15.9 ± 0.2〉

L10

TATAAT G TG CACT

17.6 ± 0.1

0.235 ± 0.003

71 ± 1

 

L04

TATAAT G AC CACT

12.3 ± 0.1

0.201 ± 0.003

58 ± 1

 

L12

TATAAT G AG CACT

0.043 ± 0.003

0.022 ± 0.003

1.9 ± 0.3

 

L16

TATAAT G AA CACT

17.5 ± 0.1

0.240 ± 0.003

69 ± 1

D 〈15.1 ± 0.2〉

L06

TATAAT G TT CACT

15.8 ± 0.1

0.219 ± 0.03

69 ± 1

 

L08

TATAAT G AT CACT

14.9 ± 0.1

0.255 ± 0.003

56 ± 1

 

L14

TATAAT G TA CACT

12.1 ± 0.1

0.304 ± 0.003

38.0 ± 0.4

 

L21

TATAAT GGGA GCT

41.6 ± 0.2

40.1 ± 0.2

0.986 ± 0.003

E

L22

GATACT GGGA GCT

0.378 ± 0.003

0.023 ± 0.004

16 ± 2

F

Ptrc1O

TATAAT GTGTG A

46.4 ± 0.2

43.9 ± 0.2

1.007 ± 0.003

G

L31

TATAAT GTGTGGT

3.08 ± 0.01

0.169 ± 0.003

17.3 ± 0.3

H

R40

GATACTGAGCACT

0.272 ± 0.003

0.082 ± 0.003

3.2 ± 0.1

I

J23101

TATTATGCTAGCTA

4.57 ± 0.02

4.461 ± 0.02

0.974 ± 0.003

n.s.

rnpB

CACACTAGAAAAAT

1.00 ± 0.01 (427 ± 2)

1.00 ± 0.01 (448 ± 2)

0.95 ± 0.01

 
  1. a, The sequences of listed promoters, excluding Ptrc1O, J23101, and rnpB promoters, are identical except the region shown in this table. The consensus -10 element TATAAT is underlined. The transcription start site (TSS) is boxed. The promoter sequences are detailed (Figure 1). b, The induced and repressed conditions are Synechocystis sp. strain ATCC27184 (i.e. glucose-tolerant Synechocystis sp. strain PCC 6803) cells in LAHG growth condition treated with and without 100 ng/mL aTc, respectively for 24 hours. The mean ± standard error of mean (s.e.m.) is relative to the strength of the rnpB promoter in the respective regulation condition. The value in parentheses is the experimental mean ± s.e.m. of EYFP emission per cell after subtracting the auto-fluorescence of Synechocystis cells containing pPMQAK1 vector only. The measurement is done by a flow cytometer to collect 50,000 events for each biological sample. c, The induction of a promoter is the ratio of its measured strength in induced compared to in repressed condition. d, The simulated thermal opening probability patterns (A-I) at 303 K are shown (Figure 4); n.s., not simulated. The value in a bracket is the mean ± s.e.m. under the induced condition of strengths of the promoters in a given pattern. The noLVA_L09 and L12 constructs are excluded in averaging. e, The only difference of noLVA_L09 to L09 is the introduction of a double stop codon in the end of the tetR gene to cease translation of a protease LVA tag tailing in C-terminal of TetR repressor. The L09 promoter regions of both are identical.