Figure 5
BioBrick™ promoters characterization, expressed in absolute units (S cell ), in the two single-copy contexts (Ф80 and aspA loci). Promoter activity was measured via the BBa_I13507 (RBS BBa_B0034-mRFP1-double terminator) part. Grey bars represent the average values, computed on at least five clones, and the error bars represent the 95% confidence intervals. For each promoter, statistical analysis was performed via t-test to compare the activities measured in the two different contexts. Comparisons showing a statistical difference (P < 0.05) in the mean activities between the two conditions are marked with a ‘+’ sign, while promoters not showing any significant difference (P ≥ 0.05) are marked with a ‘-’ sign.