Table 1 Favorite or most useful biological parts from publicly available registries

Addgene

25712:pAKTaq

Plasmid

1

Bacterial expression vector encoding the DNA polymerase from Thermus aquaticus

Coli Genetic Stock Center

CGSC #12119

Chassis

1

E. coli strain BW27783 bearing 9 known mutations

iGEM Registry

BBa_J23100 series

Regulatory

8

BBa_J23100 through BBa_J23119 is a family of constitutive promoter parts that can be used to tune the expression level of constitutively expressed parts

BBa_B0034

RBS

4

RBS based on Elowitz & Liebler repressilator

BBa_B0015

Terminator

1

Double terminator including BBa_B0010 and BBa_B0012

BBa_C0062

Coding

1

luxR repressor/activator

BBa_E2050

Coding

1

derivative of mRFP1, yeast-optimized

BBa_F2620

Signaling

1

A signaling device whereby the input is 3OC₆HSL and the output is PoPS from a LuxR-regulated operator

BBa_I15010

Coding

1

Chimeric Cph1 light receptor/EnvZ protein

BBa_I744210

Generator

1

TetR regulated LuxN-Tsr Chimeric Receptor B

BBa_J04450

Reporter

1

RFP coding device

BBa_J15001

RBS

1

strong synthetic E. coli RBS with SacI site

BBa_J153000

Plasmid Backbone

1

broad-host-range shuttle vector pPMQAK1 that provides ampicillin and kanamycin/neomycin resistance

BBa_J176005

Protein Domain

1

Codon optimized mCherry red fluorescent protein

BBa_J176006

Coding

1

Mammalian venus fluorescent protein

BBa_J176022

Protein Domain

1

Human codon-optimized AmCyan1 from pAmCyan1-C1

BBa_J33207

Reporter

1

lac promoter and lacZ

BBa_J61009

Plasmid

1

pAC-LuxGFP that places GFP under the wildtype Vibrio lux device

BBa_J64032

Device

1

pCASP SPI-1 Secretion Circuit

BBa_J85226

Composite

1

Kanamycin resistance (KanR)_off version of J85224

BBa_J176027

Regulatory

1

Constitutive cytomegalovirus promoter

BBa_J176122

Plasmid Backbone

1

pcDNA3.1 plus puromycin resistance

BBa_K566002

Regulatory

1

Biphasic switch

BBa_P1010

Generator

1

ccdB cell death gene

BBa_R0040

Regulatory

1

TetR repressible promoter