Table 2 Additional physical assembly methods identified by survey participants

Commercial/Proprietary

  Proprietary method, not specified

3

  GeneArt Seamless Assembly

2

  GeneArt High Order Assembly

1

  Clontech In-Fusion HD cloning kit

1

  Ginkgo assembly method

1

  Invitrogen TOPO cloning

1

Non-Commercial

  Conventional PCR [10, 11]

10

  Yeast in vivo recombinational cloning [12]

5

  Home-brew method, not specified

4

  Restriction-site Associated DNA (RAD) assembly [13]

2

  Anderson 2 antibiotic (2ab) assembly [14]

1

  Inverse PCR [15]

1

  Splicing by Overlap Extension (SOE) [16]

1

In development

  A new enzymatic, scarless synthesis and assembly technology

1

  Extensions of BioBytes assembly standard [17]

1