Table 2 Additional physical assembly methods identified by survey participants
From: A survey of enabling technologies in synthetic biology
| Physical Assembly Method | N |
|---|---|
| Commercial/Proprietary | |
| Proprietary method, not specified | 3 |
| GeneArt Seamless Assembly | 2 |
| GeneArt High Order Assembly | 1 |
| Clontech In-Fusion HD cloning kit | 1 |
| Ginkgo assembly method | 1 |
| Invitrogen TOPO cloning | 1 |
| Non-Commercial | |
| Conventional PCR [10, 11] | 10 |
| Yeast in vivo recombinational cloning [12] | 5 |
| Home-brew method, not specified | 4 |
| Restriction-site Associated DNA (RAD) assembly [13] | 2 |
| Anderson 2 antibiotic (2ab) assembly [14] | 1 |
| Inverse PCR [15] | 1 |
| Splicing by Overlap Extension (SOE) [16] | 1 |
| In development | |
| A new enzymatic, scarless synthesis and assembly technology | 1 |
| Extensions of BioBytes assembly standard [17] | 1 |