From: A survey of enabling technologies in synthetic biology
Physical Assembly Method | N |
---|---|
Commercial/Proprietary | |
Proprietary method, not specified | 3 |
GeneArt Seamless Assembly | 2 |
GeneArt High Order Assembly | 1 |
Clontech In-Fusion HD cloning kit | 1 |
Ginkgo assembly method | 1 |
Invitrogen TOPO cloning | 1 |
Non-Commercial | |
Conventional PCR [10, 11] | 10 |
Yeast in vivo recombinational cloning [12] | 5 |
Home-brew method, not specified | 4 |
Restriction-site Associated DNA (RAD) assembly [13] | 2 |
Anderson 2 antibiotic (2ab) assembly [14] | 1 |
Inverse PCR [15] | 1 |
Splicing by Overlap Extension (SOE) [16] | 1 |
In development | |
A new enzymatic, scarless synthesis and assembly technology | 1 |
Extensions of BioBytes assembly standard [17] | 1 |