Table 5 Technologies used by survey participants that did not self-identify as synthetic biologists (n = 17)

Physical assembly methods

  Gateway recombinatorial cloning

4

1

10

3

  de novo DNA synthesis

3

2

7

3

  Gibson assembly

3

1

3

1

  Conventional restriction site-based cloning

2

0

2

2

  CPEC

1

2

2

0

  SLIC

1

1

  PIPE

1

1

12

3

  USER

1

1

10

1

  InFusion cloning

1

0

7

2

  RAD assembly

1

0

  Transfer PCR

1

0

6

1

  Yeast in vivo cloning

1

0

4

1

  BioBrick assembly standard

0

1

3

5

Measurement, functional composition, data exchange

3

3

  RPU

0

1

2

5

  Electronic datasheets

0

1

1

2

  Visual datasheets

0

1

1

2

DNA synthesis

1

1

  Commercial, short oligos

9

4

1

1

  Commercial, gene size (>500bp)

4

3

1

1

  In-house, gene-size(>500 bp)

3

2

1

1

  In-house, short oligos

1

4

1

0

DNA sequencing

1

0

  Commercial

8

3

1

0

  In-house

2

4

1

0

Culture technique

1

0

  LB broth or agar

10

3

1

0

  37°C incubator

10

3

1

0

  Antibiotic selection

9

4

1

0

  Glycerol freezing

8

3

0

1

  30°C incubator

7

3

0

1

  Colorimetric medium

4

2

0

1

Other tools, reagents and methods

0

1

  PCR

9

5

0

1

  GFP reporters

7

4

0

1

  Non-GFP reporters

6

4

0

1

  Directed evolution

3

2