Model-driven elucidation of the inherent capacity of Geobacter sulfurreducens for electricity generation

Table 3 The identified reactions that contribute significantly to the predicted maximum NADH production rate

Reaction ID	NADH flux (mmol/gDW/h)		Enzyme	EC. No.	Reaction	Subsystems
Reaction ID	Min	Max	Enzyme	EC. No.	Reaction	Subsystems
*NADH producing reactions*
AKGD	0	35.48	2-oxoglutarate dehydrogenase	2.3.1.61	[c] : akg + coa + nad --> co2 + nadh + succoa	Central Metabolism
ALDD2x	8.855	35.32	aldehyde dehydrogenase (acetaldehyde, NAD)	1.2.1.3	[c] : acald + h2o + nad --> ac + (2) h + nadh	Amino Acid Metabolism
GCCc	4.427	17.66	part of glycine-cleavage complex	1.8.1.4	[c] : dhlpro + nad --> h + lpro + nadh	Amino Acid Metabolism
*NADH consuming reactions*
GAPD	-52.96	-0.04026	glyceraldehyde-3-phosphate dehydrogenase (NAD)	1.2.1.12	[c] : g3p + nad + pi <==> 13dpg + h + nadh	Central Metabolism
GLUSx	-52.92	0	glutamate synthase (NADH2)	1.4.1.14	[c] : akg + gln-L + h + nadh --> (2) glu-L + nad	Amino Acid Metabolism
NADH5	-10.58	0	NADH dehydrogenase (Menaquinone 7 & 2 protons)	1.6.5.3	(3) h[c] + mqn7[c] + nadh[c] --> (2) h[e] + mql7[c] + nad[c]	Energy Metabolism
*DET*
CYOR1m	35.48	35.48	cytochrome-c reductase (menaquinol 7: 1 protons)	1.7.2.2	(2) ficytcc[c] + mql7[c] --> (2) focytcc[c] + h[e] + h[c] + mqn7[c]	Energy Metabolism

(The reaction and metabolite abbreviations are listed in the Additional file 2).