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Figure 6 | Journal of Biological Engineering

Figure 6

From: Hidden hysteresis – population dynamics can obscure gene network dynamics

Figure 6

Hysteresis is affected by the dilution used for subculture. A: Hysteretic behavior in cell populations resuspended using three dilutions. Open circles show the increase in GFP intensity as a function of inducer concentration. Open triangles, diamonds and squares indicate three different dilutions. All three dilution groups showed expression levels significantly greater than the cells with no induction history at low inducer concentrations. Cell populations resuspended using the lowest dilution (Curve A with 1:1 dilution) generally showed the highest expression levels upon reaching steady-state, and populations resuspended using the highest dilution (Curve C, 1:100) showed the lowest. Therefore, the overall magnitude of the population-level hysteresis depends on the dilution used during resuspension, and hysteresis may not be observable within a population if very high dilutions are used during resuspension. Note: error bars represent ±2 standard deviations. B: Hysteresis quantified in terms of proportion of cells in the ON state. The trends for the three dilutions closely resemble trends seen in relative fluorescence.

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