Figure 4

Expression analysis for lysates of E. coli strain FTD147 with no hydrogenase construct (NC), over-expressing wild type A. macleodii hydrogenase enzyme (WT), or H230C, P285C, and H230C/P285C substitutions, and wild type hydrogenase subjected to thermal denaturation (WT TD). Before loading, proteins were normalized for protein content at 5 mg mL^-1. Associated enzyme activites can be found in Figure S1. Top: Sypro-Ruby stain of the gel, indicating approximately equivalent amounts of protein loaded per lane. Bottom: anti-HynL western blot of samples.