Figure 4

Heat maps illustrating the effect of switching the location of specific ORFs relative to the driving promoter. Maps include all possible tagging combinations for 24 bi-cistronic operons sharing tags Avi, HSV and T7. The top two maps illustrate patterns of expression for a set of constructs where Med7 and Med 21 are located in the first and second ORF positions, respectively. The bottom two maps illustrates patterns of expression for the same set of epitope tags only that Med7 and Med 21 are now located in the second and first ORF positions, respectively. Maps on the left quantify expression levels for the first ORF (in the “y” axis) and should be read from left to right, whereas maps on the right quantify expression levels from the second ORF (in the “x” axis) and should be read from top to bottom. As before, red and yellow colors indicate high and low relative expression levels, respectively.