2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

Table 1 Summary of BglBricks-based 2ab assembly results

Assembly stage	1			2		3	Totals
junction type	CA	AK	KC	CK	KA	CA
junctions made	28	14	14	24	24	528	632
colonies screened by growth phenotype	84	42	42	156	156	1728	2208
co-transformants	3	1	11	12	7	77	111
% co-transformation	3.57	2.38	26.19	7.69	4.49	4.46	5.03
colonies screened by mapping or PCR	28	14	14	48	48	622	774
colonies w/ correct map or PCR phenotype	26	13	13	47	48	535	682
colonies w/ questionable map or PCR phenotype	2	1	1	0	0	75	79
colonies w/ incorrect map or PCR phenotype	0	0	0	1	0	12	13
colonies sequenced	9	1	14	9	8	73	114
colonies w/ correct map or PCR sequenced	7	1	13	9	8	65	103
correct sequencing result	7	1	13	9	8	65	103
colonies w/ questionable map or PCR sequenced	2	1	1	n/a	n/a	4	8
correct sequencing result	0	1	0	n/a	n/a	3	4
colonies w/ incorrect map or PCR sequenced	n/a	n/a	n/a	0	n/a	4	4
correct sequencing result	n/a	n/a	n/a	n/a	n/a	0	0
% correct assembly	92.86	100	92.86	97.92	100	95.06	96.45*

ISSN: 1754-1611