From: 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
Assembly stage | 1 | Totals | |||||
---|---|---|---|---|---|---|---|
junction type | CA | AK | KC | CK | KA | AC | Â |
total junctions made | 1 | 1 | 1 | 1 | 1 | 1 | 6 |
colonies screened by growth phenotype | 200 | 198 | 201 | 200 | 199 | 201 | 1199 |
co-transformants | 29 | 26 | 27 | 37 | 24 | 31 | 174 |
% co-transformation | 14.50 | 13.13 | 13.43 | 18.5 | 12.06 | 15.42 | 14.51 |
total colonies counted | 580 | 1011 | 274 | 366 | 688 | 567 | 3486 |
white colonies | 45 | 83 | 24 | 34 | 48 | 31 | 265 |
green colonies | 535 | 928 | 250 | 332 | 640 | 536 | 3221 |
total colonies sequenced | 5 | 6 | 5 | 2 | 7 | 1 | 26 |
white colonies sequenced | 5 | 6 | 5 | 2 | 7 | 1 | 26 |
correct sequencing result | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
green colonies sequenced | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
correct sequencing result | n/a | n/a | n/a | n/a | n/a | n/a | 0 |
% correct assembly | 92.24 | 91.79 | 91.24 | 90.71 | 93.02 | 94.53 | 92.40 |