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Table 2 Summary of BBF RFC10-based 2ab assembly results

From: 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

Assembly stage

1

Totals

junction type

CA

AK

KC

CK

KA

AC

 

total junctions made

1

1

1

1

1

1

6

colonies screened by growth phenotype

200

198

201

200

199

201

1199

co-transformants

29

26

27

37

24

31

174

% co-transformation

14.50

13.13

13.43

18.5

12.06

15.42

14.51

total colonies counted

580

1011

274

366

688

567

3486

white colonies

45

83

24

34

48

31

265

green colonies

535

928

250

332

640

536

3221

total colonies sequenced

5

6

5

2

7

1

26

white colonies sequenced

5

6

5

2

7

1

26

correct sequencing result

0

0

0

0

0

0

0

green colonies sequenced

0

0

0

0

0

0

0

correct sequencing result

n/a

n/a

n/a

n/a

n/a

n/a

0

% correct assembly

92.24

91.79

91.24

90.71

93.02

94.53

92.40