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Figure 4 | Journal of Biological Engineering

Figure 4

From: Targeting a heterologous protein to multiple plant organelles via rationally designed 5′ mRNA tags

Figure 4

Typical transient expression pattern controls in N. benthamiana leaf epidermal cells. GFP channel (a, d, g), chlorophyll autofluorescence (b, e, h) and composite (c, f, i) images were generated using confocal microscopy. Comparison with a,b and c allow for identification of non-targeted GFP by a pattern of cytosolic, plasma membrane and nuclear expression. The PIMT2-GFP fusion in d, e and f shows expression in the chloroplast. The TTL-GFP fusion in g, h, and i shows expression throughout the cell but some preference for small organelles we believe to be peroxisomes (arrow). Scale bar is 25 μm.

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