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Table 1 Production of PHB in secreting (pCMEL3 + pLG575) and non-secreting (pBHR68 + pLG575) strains of E. coli at 24 and 48 h

From: Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach

  

% mass of PHB in dry mass

Production g/L PHB

 

Strain

Time (h)

Secreted fraction

Non- secreted fraction

Secreted fraction

Non-secreted fraction

Total production

g PHB/g Glucose

Non-secreting

24

0.31 ± 0.35

41.93 ± 13.5

0.40 ± 0.27

5.65 ± 1.1

6.05 ± 1.1

0.40 ± 0.07

 

48

0.72 ± 0.89

47.24 ± 6.0

0.50 ± 0.41

5.43 ± 1.7

5.93 ± 1.8

0.40 ± 0.12

Secreting

24

0.69 ± 0.18

28.85 ± 0.41

0.40 ± 0.06

3.42 ± 0.33

3.82 ± 0.3

0.25 ± 0.02

 

48

28.29 ± 7.2*

38.80 ± 15.5

2.57 ± 0.75*

4.58 ± 2.47

7.15 ± 2.6

0.48 ± 0.17

  1. *Indicates statistical significance within column (p < 0.05).