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Figure 5 | Journal of Biological Engineering

Figure 5

From: Engineering biodegradable and multifunctional peptide-based polymers for gene delivery

Figure 5

Comparative transfection of p [HPMA- co -K 10 ] polymers (“pHK10”) modified with various endosomal escape modalities in HeLa cells. HeLa cells (3 × 104) were treated with polyplexes (containing 1 μg plasmid DNA) at charge ratios (N/P) of 3, 4, and 5 for 4 h in serum-free conditions, washed, and replenished with complete media. At 48 h post-transfection, cell lysates were assessed for (A) luciferase reporter gene expression and (B) protein content as an indicator for cytotoxicity. pHK10 unmodified: p[HPMA-co-K10]; pHK10 + histidine: p[HPMA-co-K10-co-K5H5] [55]; pHK10 mix + 5% melittin: a mixture of 95:5% (v/v) p[HPMA-co-K10]: p[HPMA-co-melittin]-b-[HPMA-co-K10] [56]; pHK10 mix + 15% melittin: a mixture of 85:15% (v/v) p[HPMA-co-K10]: p[HPMA-co-melittin]-b-[HPMA-co-K10]; pHK10 + melittin: p[HPMA-co-melittin]-b-[HPMA-co-K10]; pHK10 + st-sHGP: p[HPMA-co-K10-co-sHGP]; pHK10 + b-sHGP: p[HPMA-co-sHGP]-b-[HPMA-co-K10]. Data are presented as the mean ± S.D., n = 3.

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