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Figure 1 | Journal of Biological Engineering

Figure 1

From: Design and analysis of a tunable synchronized oscillator

Figure 1

Scheme of the basic synchronized oscillator consisting of modified lux quorum sensing machinery, aiiA, and GFP. LuxI produces AHL, which forms a complex with the constitutively expressed LuxR. This complex induces further expression of LuxI (positive feedback loop depicted in blue) as well as AiiA, which in turn degrades AHL (negative feedback loop depicted in red). The expression of GFP is dependent on the AHL concentration present in the system and thus serves as a reporter of the oscillating AHL levels. The three AHL inducible promoters are derived from the lux promoter. Those depicted in blue and yellow are also repressible by corresponding transcription factors, but behave identically in absence of them.

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