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Figure 2 | Journal of Biological Engineering

Figure 2

From: Design and analysis of a tunable synchronized oscillator

Figure 2

Circuit expanded with repressors enables tuning of the feedback kinetics. The transcriptional repressors TetR and LacI and the red fluorescent reporter mCherry are expressed as a contiguous transcript under control of the araC/pBAD promoter. TetR and LacI repress the transcription of aiiA and luxI, respectively. The presence of the inducer molecules aTc and IPTG relieve the repression as a function of their intracellular concentrations, effectively modulating the strength of the oscillating components’ transcriptional feedback.

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