Figure 2From: Design and analysis of a tunable synchronized oscillatorCircuit expanded with repressors enables tuning of the feedback kinetics. The transcriptional repressors TetR and LacI and the red fluorescent reporter mCherry are expressed as a contiguous transcript under control of the araC/pBAD promoter. TetR and LacI repress the transcription of aiiA and luxI, respectively. The presence of the inducer molecules aTc and IPTG relieve the repression as a function of their intracellular concentrations, effectively modulating the strength of the oscillating components’ transcriptional feedback.Back to article page