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Figure 1 | Journal of Biological Engineering

Figure 1

From: Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MAT a/α diploids

Figure 1

Schematic outline of experimental design. (A) Isolation of a-type of cells generated via spontaneous chromosomal aberration. P PGK1 -a1 suppresses mating ability of α-type of cells to prevent autopolyploidization (mating between a- and α-type of cells derived from the same parental strain). The URA3 gene is expressed only in a-type cells, and a-type of cells would be selected on SD solid medium lacking uracil (SD – Ura plates). (B) Isolation of α-type of cells generated via spontaneous chromosomal aberration. P STE2 -α2 suppresses mating ability of a-type cells to prevent autopolyploidization. The URA3 gene is expressed only in α-type cells, and α-type of cells would be selected on SD – Ura plates. The GFP reporter gene is used to detect yeast cells with target mating-type.

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