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Figure 2 | Journal of Biological Engineering

Figure 2

From: Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MAT a/α diploids

Figure 2

Growth of yeast transformants harboring YEp-type plasmids. (A) Plasmid map of pHY-2U containing 2 μ origin of replication (providing cellular retention of multicopy plasmids) and P STE2 -URA3 construct (activated in a-type yeast cells). (B) The OD600 values of cultures of pHY-2U transformants at 18 h cultivation. Black bars indicate cultivation with uracil, and gray bars indicate cultivation without uracil. Values are presented as means ± standard deviations from three independent experiments. (C) Plasmid map of pLY-3U containing 2 μ origin of replication and P STE3 -URA3 construct (activated in α-type yeast cells). (D) The OD600 values of cultures of pLY-3U transformants at 18 h cultivation. Symbols are as in B.

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Journal of Biological Engineering

ISSN: 1754-1611