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Figure 3 | Journal of Biological Engineering

Figure 3

From: Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MAT a/α diploids

Figure 3

Growth of yeast transformants harboring YCp-type of plasmids. (A) Plasmid map of pLS-2U containing CEN6/ARSH4 origin of replication (providing cellular retention of single-copy plasmids) and P STE2 -URA3 construct (activated in a-type yeast cells). (B) The growth curves of pLS-2U transformants. Closed symbols indicate cultivation with uracil, and open symbols indicate cultivation without uracil. Values are presented as means ± standard deviations from three independent experiments. (C) Plasmid map of pHS-3U containing CEN6/ARSH4 origin of replication and P STE3 -URA3 contruct (activated in α-type yeast cells). (D) The growth curves of pHS-3U transformants. Symbols are as in B.

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Journal of Biological Engineering

ISSN: 1754-1611