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Figure 4 | Journal of Biological Engineering

Figure 4

From: Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MAT a/α diploids

Figure 4

Improved growth selection system for α-type of cells. (A) Plasmids used for α-type-specific URA3 gene expression. The plasmid pHS-HoU contains CEN6/ARSH4 origin of replication (providing cellular retention of single-copy plasmids) and P HO -URA3 construct (activated in yeast haploid cells). The plasmid pL3G-2α contains P STE2 -α2, a construct that represses URA3 gene expression in a-type yeast cells. (B) The growth curves of double plasmid (pHS-HoU and pL3G-2α) transformants. Closed symbols indicate cultivation with uracil, and open symbols indicate cultivation without uracil. Values are presented as means ± standard deviations from three independent experiments.

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Journal of Biological Engineering

ISSN: 1754-1611