Skip to main content
Figure 5 | Journal of Biological Engineering

Figure 5

From: Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MAT a/α diploids

Figure 5

Schematic outline of yeast mating-type regulation and our engineered approach. (A) Engineering for isolation of a-type of cells. The MAT a/α parental strain indicates BY4743 harboring pH2G-Pa1 and pLS-2U (see Table 1). (B) Engineering for isolation of α-type of cells. The MAT a/α parental strain indicates BY4743 harboring pL3G-α2 and pHS-HoU (see Table 1). Chromosomal aberration regarding the MAT locus induces generation of two kinds of yeast cells, i.e., a- and α-type of cells. Here we described yeast cells possessing two sets of MAT genes (generated via LOH) as an example. Then, spontaneous or engineered formation of the a1-α2 complex suppresses the mating response by repressing expression of haploid-specific genes (hsg), which encode components of the mating signaling pathway. Target cells expressing the URA3 selection marker gene can be isolated on SD – Ura plates in the absence of autopolyploidization. The target cells also express the GFP reporter gene. Regulation colored with cyan indicates indirect gene repression; the a1-α2 complex prevents expression of transcriptional activator α1 that is required for gene expression under the control of P STE3 . Note that URA3 gene is directly repressed by a transcriptional repressor in non-target cells.

Back to article page