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Table 1 Yeast strains and plasmids used in this study

From: Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MAT a/α diploids

Name

Description

Reference source

Yeast strains

  

BY4741

MAT a his3 Δ1 ura3 Δ0 leu2 Δ0 met15 Δ0

Brachmann et al.,[24]

BY4742

MAT α his3 Δ1 ura3 Δ 0 leu2 Δ0 lys2 Δ0

Brachmann et al.,[24]

BY4743

MAT a / α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0

Brachmann et al.,[24]

BY4743A

MAT a his3Δ1 leu2Δ0 LYS2 MET15 ura3Δ0

Present study

BY4743AL

MAT α / α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0

Present study

Plasmids

  

pRS313

Yeast expression vector; CEN6/ARSH4 ori and HIS3 marker

NBRP*

pRS315

Yeast expression vector; CEN6/ARSH4 ori and LEU2 marker

NBRP

pRS316

Yeast expression vector; CEN6/ARSH4 ori and URA3 marker

NBRP

pHY-2GA

Yeast expression vector; 2 μ ori, HIS3 marker and P STE2 -EGFP

Fukuda et al.,[18]

pLY-3GC

Yeast expression vector; 2 μ ori, LEU2 marker and P STE3 -EGFP

Fukuda et al.,[18]

pH2G-Pa1

P PGK1 - a1 in pHY-2GA

Fukuda et al.,[18]

pL3G-2α

P STE2 - α2 in pLY-3GC

Fukuda et al.,[18]

pHY-2U

Yeast expression vector; 2 μ ori, HIS3 marker and P STE2 -URA3

Present study

pLY-3U

Yeast expression vector; 2 μ ori, LEU2 marker and P STE3 - URA3

Present study

pLS-2U

P STE2 -URA3 in pYO315

Present study

pHS-3U

P STE3 -URA3 in pYO313

Present study

pHS-HoU

P HO -URA3 in pYO313

Present study

  1. *Resources were provided by the National Bio-Resource Project (NBRP) of the MEXT, Japan.