Figure 3

Luciferase activities of the constitutive Bacillus BioBrick Box promoters. Measurement of promoter activity was done based on pBS3Clux-derivatives via luminescence of 100 μl cultures growing in a plate reader (Biotek®, Synergy™2; 96-well plate, 37°C, shaking) over time. The graphs are aligned to an OD₆₀₀ = 0.1 at time point 0 (grey line). (A)-(C) show data of growth in complex LB medium; (D)-(F) show data of growth in the defined medium MCSE. (A) and (D) Growth curves (OD₆₀₀) from cultures of the reporter strains TMB1872 (lux without promoter), TMB1862 (J23101-lux), TMB1856 (P _liaG -lux), TMB1860 (P _lepA -lux) and TMB1930 (P _veg -lux). (B) and (E) Promoter activities of the same strains expressed as luminescence in relative luminescence units (RLU) per OD₆₀₀ and using the same color code as in panels (A) and (D). (C) and (F) Bar graphs of relative promoter units (RPUs, normalized to P _liaG ) at time point 0, corresponding to OD₆₀₀ = 0.1. All graphs show mean values and standard deviations (error bars) of at least four biological replicates.