Luciferase activities of the constitutive Bacillus BioBrick Box promoters. Measurement of promoter activity was done based on pBS3Clux-derivatives via luminescence of 100 μl cultures growing in a plate reader (Biotek®, Synergy™2; 96-well plate, 37°C, shaking) over time. The graphs are aligned to an OD600 = 0.1 at time point 0 (grey line). (A)-(C) show data of growth in complex LB medium; (D)-(F) show data of growth in the defined medium MCSE. (A) and (D) Growth curves (OD600) from cultures of the reporter strains TMB1872 (lux without promoter), TMB1862 (J23101-lux), TMB1856 (P
-lux), TMB1860 (P
-lux) and TMB1930 (P
-lux). (B) and (E) Promoter activities of the same strains expressed as luminescence in relative luminescence units (RLU) per OD600 and using the same color code as in panels (A) and (D). (C) and (F) Bar graphs of relative promoter units (RPUs, normalized to P
) at time point 0, corresponding to OD600 = 0.1. All graphs show mean values and standard deviations (error bars) of at least four biological replicates.