Figure 5
Luciferase activity of the xylose-inducible P xylA promoter. Measurement of Pxyl-activity in pBS3Clux upon induction with various concentrations (see legend) of xylose. Addition of water was used as non-induced reference. For details of growth and measurement conditions, see legend of Figure 3 and methods. (A)-(C) Growth in LB (D)-(F) Growth in MCSE. (A) and (D) Growth curves (OD600) from cultures of the reporter strain TMB1931 (P xylA -lux) in semi-logarithmic scale. (B) and (E) Promoter activities upon xylose-induction expressed as luminescence in RLU/OD600. (C) and (F) Dose–response curves one hour post-induction in RPUs (normalized to P liaG ). All graphs show mean values and standard deviations (error bars) of at least four biological replicates.