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Figure 1 | Journal of Biological Engineering

Figure 1

From: Assembly of eukaryotic algal chromosomes in yeast

Figure 1

Cloning of P. tricornutum chromosomes as five overlapping fragments. A. Chromosomes 25 and 26 were each cloned as five overlapping fragments in yeast using a BAC-YAC vector. Fragments were further modified by addition of the URA3 selectable marker in fragment 3 (asterisk) and the addition of a yeast replication origin (ARS) in fragments 2, 3, and 4 (vertical bar). B and C. The cloned fragments were purified from yeast, transformed into E. coli, and were purified and separated on agarose gels as supercoiled plasmids. Chromosome 25 (B) and 26 (C) fragments were each in the following order: BAC-Tracker supercoiled DNA ladder (Epicentre, lane 1), Fragment 1 (lane 2), Fragment 2 (larger version ~ 170 kb, lane 3), Fragment 2-ARS (lane 4), Fragment 3-URA (lane 5), Fragment 3-URA-ARS (lane 6), Fragment 4 (lane 7), Fragment 4-ARS (lane 8), Fragment 5 (lane 9), BAC-Tracker ladder (lane 10).

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