Figure 2

Summary of assembly efficiency for chromosomes 25 and 26. Assembly of each of P. tricornutum chromosomes 25 (A and B) and 26 (C and D) from five overlapping fragments was performed using yeast spheroplast transformation. Fragments to assemble were modified with the URA3 selectable marker in the third fragment only (A and C) or URA3 selectable marker in the third fragment and yeast origins of replication in fragments 2, 3, and 4 (B and D). One hundred resulting colonies were screened by low resolution multiplex PCR (one amplicon per fragment), and the number of fragments cloned for each assembly was plotted.