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Table 3 Sites of insertion of yeast replication origins (KmR-ARS) and yeast selectable markers (URA) in cloned P. tricornutum fragments

From: Assembly of eukaryotic algal chromosomes in yeast

Fragment

KmR-ARS insertion position (nt from beginning of chromosome sequence at 5’ site of insertion)

URA insertion position (nt from beginning of chromosome sequence at 5’ site of insertion)

25-2

120000

NA

25-3

241000

239990

25-4

357000

NA

26-2

115973

NA

26-3

230089

213353

26-4

358818

NA