Figure 5

Raw color images (left column) and corresponding ratiometric images (right column) of cotton bolls with various types of damage and stages of infestation. (A) and (B) show a non-infested cotton boll without any exterior damage marks. (C) and (D) show a cotton boll punctured by a sterilized syringe for negative control studies. The black ring in (C) was drawn with a fiber tip marker. (E) and (F) show an example of a control cotton boll that exhibits fluorescent marks very similar to stink bug puncture marks. Those marks would typically be identified as positives and count as false positives. (G) and (H) are images of a cotton boll with exterior puncture marks caused by stink bug feeding. The marks are visible as larger fluorescent green regions and count as true positives. Note the presence of smaller fluorescent dots not caused by stink bugs. The scale bar (white line in lower left corner of image A) represents 5 mm.