Figure 9

Receiver operating characteristic (ROC) for different-size rectangles ( cf. Figure8) as a study how well a dual threshold criterion can separate true and false positive fluorescent marks. As specific corner points for the rectangle in Figure 8 were chosen: 1: (1.5, 1.4), 2: (1.0, 1.2), 3: (0.8, 1.1), 4: (0.65, 1.05), 5: (0.5, 1.0), 6: (0.4, 0.98). At point 1, sensitivity is high but specificity is low, and this changes towards the higher point number. At point 6 we reach lower values for sensitivity but higher values for specificity. Striking a balance in this instance includes reducing the number of false positives (high sensitivity) and false negatives (high specificity). Dashed line represents a random assumption.